r d systems cd200 2724 cd 050 Search Results


recombinant mouse cd200 fc chimera protein, cf  (Bio-Techne corporation)
93
Bio-Techne corporation recombinant mouse cd200 fc chimera protein, cf
Recombinant Mouse Cd200 Fc Chimera Protein, Cf, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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recombinant mouse cd200 fc chimera protein, cf - by Bioz Stars, 2026-10
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r d systems cd200 2724 cd 050  (R&D Systems)
93
R&D Systems r d systems cd200 2724 cd 050
R D Systems Cd200 2724 Cd 050, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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‒1 cd200 fc chimaera protein  (R&D Systems)
91
R&D Systems ‒1 cd200 fc chimaera protein
Upregulated <t>CD200</t> may contribute to decreased osteoclastogenic activity in Gli1-Cre ERT2 ;Bmpr1a fl/fl sutures. a FACS analysis of CD200 in suture mesenchymal cells collected from Bmpr1α fl/fl (control) and Gli1-Cre ERT2 ;Bmpr1α fl/fl (mutant) mice 2 weeks post induction. b Quantitation of the percentages of CD200+ cells in suture mesenchyme from three independent samples. c Immunostaining of CD200 (green, indicated by arrows) in the suture mesenchyme of Bmpr1α fl/fl (control) and Gli1-Cre ERT2 ;Bmpr1α fl/fl (mutant) mice 2 weeks post induction (2wpt). Broken lines indicate the outline of the suture. d TRAP staining of osteoclasts induced from BMMs of 4-week-old C57BL/6J mice, after culture with M-CSF for 3 days and then with RANKL or RANKL plus exogenous CD200 for another 5 days. The inset shows a magnified view of the boxed region. Blue arrows indicate mature osteoclasts. e Quantitation of multinucleated TRAP+ osteoclasts per well in four independent experiments. f Resorption activity assay (von Kossa staining) of osteoclasts induced from BMMs of 4-week-old C57BL/6J mice, after culture with M-CSF for 3 days and then with RANKL or RANKL plus exogenous CD200 for another 5 days. g Quantitation of the percentage of resorption in four independent experiments. h Real-time PCR of hedgehog signalling members ( Ihh , Ptch1 , and Gli1 ), osteogenic markers ( Alp , Runx2 , and Sp7 ), and osteoclastic-related markers ( CD200 , CD200R , OPG , and RANKL ) in the suture mesenchyme of C57BL/6J mice 2 weeks post treatment with or without GDC0449 (hedgehog inhibitor) from five independent samples. T tests were performed. * P < 0.05; ** P < 0.01. Scale bars, 100 µm
‒1 Cd200 Fc Chimaera Protein, supplied by R&D Systems, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/r+d+systems+cd200+2724+cd+050/Recombinant+Human+CD200+Fc+Chimera+Protein%2C+CF/pmc06193039-276-14-19
Average 91 stars, based on 1 article reviews
‒1 cd200 fc chimaera protein - by Bioz Stars, 2026-10
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recombinant human igg1 fc protein, cf  (Bio-Techne corporation)
96
Bio-Techne corporation recombinant human igg1 fc protein, cf
Upregulated <t>CD200</t> may contribute to decreased osteoclastogenic activity in Gli1-Cre ERT2 ;Bmpr1a fl/fl sutures. a FACS analysis of CD200 in suture mesenchymal cells collected from Bmpr1α fl/fl (control) and Gli1-Cre ERT2 ;Bmpr1α fl/fl (mutant) mice 2 weeks post induction. b Quantitation of the percentages of CD200+ cells in suture mesenchyme from three independent samples. c Immunostaining of CD200 (green, indicated by arrows) in the suture mesenchyme of Bmpr1α fl/fl (control) and Gli1-Cre ERT2 ;Bmpr1α fl/fl (mutant) mice 2 weeks post induction (2wpt). Broken lines indicate the outline of the suture. d TRAP staining of osteoclasts induced from BMMs of 4-week-old C57BL/6J mice, after culture with M-CSF for 3 days and then with RANKL or RANKL plus exogenous CD200 for another 5 days. The inset shows a magnified view of the boxed region. Blue arrows indicate mature osteoclasts. e Quantitation of multinucleated TRAP+ osteoclasts per well in four independent experiments. f Resorption activity assay (von Kossa staining) of osteoclasts induced from BMMs of 4-week-old C57BL/6J mice, after culture with M-CSF for 3 days and then with RANKL or RANKL plus exogenous CD200 for another 5 days. g Quantitation of the percentage of resorption in four independent experiments. h Real-time PCR of hedgehog signalling members ( Ihh , Ptch1 , and Gli1 ), osteogenic markers ( Alp , Runx2 , and Sp7 ), and osteoclastic-related markers ( CD200 , CD200R , OPG , and RANKL ) in the suture mesenchyme of C57BL/6J mice 2 weeks post treatment with or without GDC0449 (hedgehog inhibitor) from five independent samples. T tests were performed. * P < 0.05; ** P < 0.01. Scale bars, 100 µm
Recombinant Human Igg1 Fc Protein, Cf, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
recombinant human igg1 fc protein, cf - by Bioz Stars, 2026-10
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purified mouse anti-human cd200  (Becton Dickinson)
90
Becton Dickinson purified mouse anti-human cd200
Upregulated <t>CD200</t> may contribute to decreased osteoclastogenic activity in Gli1-Cre ERT2 ;Bmpr1a fl/fl sutures. a FACS analysis of CD200 in suture mesenchymal cells collected from Bmpr1α fl/fl (control) and Gli1-Cre ERT2 ;Bmpr1α fl/fl (mutant) mice 2 weeks post induction. b Quantitation of the percentages of CD200+ cells in suture mesenchyme from three independent samples. c Immunostaining of CD200 (green, indicated by arrows) in the suture mesenchyme of Bmpr1α fl/fl (control) and Gli1-Cre ERT2 ;Bmpr1α fl/fl (mutant) mice 2 weeks post induction (2wpt). Broken lines indicate the outline of the suture. d TRAP staining of osteoclasts induced from BMMs of 4-week-old C57BL/6J mice, after culture with M-CSF for 3 days and then with RANKL or RANKL plus exogenous CD200 for another 5 days. The inset shows a magnified view of the boxed region. Blue arrows indicate mature osteoclasts. e Quantitation of multinucleated TRAP+ osteoclasts per well in four independent experiments. f Resorption activity assay (von Kossa staining) of osteoclasts induced from BMMs of 4-week-old C57BL/6J mice, after culture with M-CSF for 3 days and then with RANKL or RANKL plus exogenous CD200 for another 5 days. g Quantitation of the percentage of resorption in four independent experiments. h Real-time PCR of hedgehog signalling members ( Ihh , Ptch1 , and Gli1 ), osteogenic markers ( Alp , Runx2 , and Sp7 ), and osteoclastic-related markers ( CD200 , CD200R , OPG , and RANKL ) in the suture mesenchyme of C57BL/6J mice 2 weeks post treatment with or without GDC0449 (hedgehog inhibitor) from five independent samples. T tests were performed. * P < 0.05; ** P < 0.01. Scale bars, 100 µm
Purified Mouse Anti Human Cd200, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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recombinant human mmp11 (catalytic domain  (Enzo Biochem)
90
Enzo Biochem recombinant human mmp11 (catalytic domain
Upregulated <t>CD200</t> may contribute to decreased osteoclastogenic activity in Gli1-Cre ERT2 ;Bmpr1a fl/fl sutures. a FACS analysis of CD200 in suture mesenchymal cells collected from Bmpr1α fl/fl (control) and Gli1-Cre ERT2 ;Bmpr1α fl/fl (mutant) mice 2 weeks post induction. b Quantitation of the percentages of CD200+ cells in suture mesenchyme from three independent samples. c Immunostaining of CD200 (green, indicated by arrows) in the suture mesenchyme of Bmpr1α fl/fl (control) and Gli1-Cre ERT2 ;Bmpr1α fl/fl (mutant) mice 2 weeks post induction (2wpt). Broken lines indicate the outline of the suture. d TRAP staining of osteoclasts induced from BMMs of 4-week-old C57BL/6J mice, after culture with M-CSF for 3 days and then with RANKL or RANKL plus exogenous CD200 for another 5 days. The inset shows a magnified view of the boxed region. Blue arrows indicate mature osteoclasts. e Quantitation of multinucleated TRAP+ osteoclasts per well in four independent experiments. f Resorption activity assay (von Kossa staining) of osteoclasts induced from BMMs of 4-week-old C57BL/6J mice, after culture with M-CSF for 3 days and then with RANKL or RANKL plus exogenous CD200 for another 5 days. g Quantitation of the percentage of resorption in four independent experiments. h Real-time PCR of hedgehog signalling members ( Ihh , Ptch1 , and Gli1 ), osteogenic markers ( Alp , Runx2 , and Sp7 ), and osteoclastic-related markers ( CD200 , CD200R , OPG , and RANKL ) in the suture mesenchyme of C57BL/6J mice 2 weeks post treatment with or without GDC0449 (hedgehog inhibitor) from five independent samples. T tests were performed. * P < 0.05; ** P < 0.01. Scale bars, 100 µm
Recombinant Human Mmp11 (Catalytic Domain, supplied by Enzo Biochem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/r+d+systems+cd200+2724+cd+050/recombinant+human+mmp20+catalytic+domain/pmc09621138__jci___132___150750___s008-9-29-33
Average 90 stars, based on 1 article reviews
recombinant human mmp11 (catalytic domain - by Bioz Stars, 2026-10
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dmem high glucose  (Thermo Fisher)
99
Thermo Fisher dmem high glucose
Upregulated <t>CD200</t> may contribute to decreased osteoclastogenic activity in Gli1-Cre ERT2 ;Bmpr1a fl/fl sutures. a FACS analysis of CD200 in suture mesenchymal cells collected from Bmpr1α fl/fl (control) and Gli1-Cre ERT2 ;Bmpr1α fl/fl (mutant) mice 2 weeks post induction. b Quantitation of the percentages of CD200+ cells in suture mesenchyme from three independent samples. c Immunostaining of CD200 (green, indicated by arrows) in the suture mesenchyme of Bmpr1α fl/fl (control) and Gli1-Cre ERT2 ;Bmpr1α fl/fl (mutant) mice 2 weeks post induction (2wpt). Broken lines indicate the outline of the suture. d TRAP staining of osteoclasts induced from BMMs of 4-week-old C57BL/6J mice, after culture with M-CSF for 3 days and then with RANKL or RANKL plus exogenous CD200 for another 5 days. The inset shows a magnified view of the boxed region. Blue arrows indicate mature osteoclasts. e Quantitation of multinucleated TRAP+ osteoclasts per well in four independent experiments. f Resorption activity assay (von Kossa staining) of osteoclasts induced from BMMs of 4-week-old C57BL/6J mice, after culture with M-CSF for 3 days and then with RANKL or RANKL plus exogenous CD200 for another 5 days. g Quantitation of the percentage of resorption in four independent experiments. h Real-time PCR of hedgehog signalling members ( Ihh , Ptch1 , and Gli1 ), osteogenic markers ( Alp , Runx2 , and Sp7 ), and osteoclastic-related markers ( CD200 , CD200R , OPG , and RANKL ) in the suture mesenchyme of C57BL/6J mice 2 weeks post treatment with or without GDC0449 (hedgehog inhibitor) from five independent samples. T tests were performed. * P < 0.05; ** P < 0.01. Scale bars, 100 µm
Dmem High Glucose, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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glutamax  (Thermo Fisher)
99
Thermo Fisher glutamax
Upregulated <t>CD200</t> may contribute to decreased osteoclastogenic activity in Gli1-Cre ERT2 ;Bmpr1a fl/fl sutures. a FACS analysis of CD200 in suture mesenchymal cells collected from Bmpr1α fl/fl (control) and Gli1-Cre ERT2 ;Bmpr1α fl/fl (mutant) mice 2 weeks post induction. b Quantitation of the percentages of CD200+ cells in suture mesenchyme from three independent samples. c Immunostaining of CD200 (green, indicated by arrows) in the suture mesenchyme of Bmpr1α fl/fl (control) and Gli1-Cre ERT2 ;Bmpr1α fl/fl (mutant) mice 2 weeks post induction (2wpt). Broken lines indicate the outline of the suture. d TRAP staining of osteoclasts induced from BMMs of 4-week-old C57BL/6J mice, after culture with M-CSF for 3 days and then with RANKL or RANKL plus exogenous CD200 for another 5 days. The inset shows a magnified view of the boxed region. Blue arrows indicate mature osteoclasts. e Quantitation of multinucleated TRAP+ osteoclasts per well in four independent experiments. f Resorption activity assay (von Kossa staining) of osteoclasts induced from BMMs of 4-week-old C57BL/6J mice, after culture with M-CSF for 3 days and then with RANKL or RANKL plus exogenous CD200 for another 5 days. g Quantitation of the percentage of resorption in four independent experiments. h Real-time PCR of hedgehog signalling members ( Ihh , Ptch1 , and Gli1 ), osteogenic markers ( Alp , Runx2 , and Sp7 ), and osteoclastic-related markers ( CD200 , CD200R , OPG , and RANKL ) in the suture mesenchyme of C57BL/6J mice 2 weeks post treatment with or without GDC0449 (hedgehog inhibitor) from five independent samples. T tests were performed. * P < 0.05; ** P < 0.01. Scale bars, 100 µm
Glutamax, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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na plastic equipment greiner bio one 6 well culture plate  (Greiner Bio)
96
Greiner Bio na plastic equipment greiner bio one 6 well culture plate
Upregulated <t>CD200</t> may contribute to decreased osteoclastogenic activity in Gli1-Cre ERT2 ;Bmpr1a fl/fl sutures. a FACS analysis of CD200 in suture mesenchymal cells collected from Bmpr1α fl/fl (control) and Gli1-Cre ERT2 ;Bmpr1α fl/fl (mutant) mice 2 weeks post induction. b Quantitation of the percentages of CD200+ cells in suture mesenchyme from three independent samples. c Immunostaining of CD200 (green, indicated by arrows) in the suture mesenchyme of Bmpr1α fl/fl (control) and Gli1-Cre ERT2 ;Bmpr1α fl/fl (mutant) mice 2 weeks post induction (2wpt). Broken lines indicate the outline of the suture. d TRAP staining of osteoclasts induced from BMMs of 4-week-old C57BL/6J mice, after culture with M-CSF for 3 days and then with RANKL or RANKL plus exogenous CD200 for another 5 days. The inset shows a magnified view of the boxed region. Blue arrows indicate mature osteoclasts. e Quantitation of multinucleated TRAP+ osteoclasts per well in four independent experiments. f Resorption activity assay (von Kossa staining) of osteoclasts induced from BMMs of 4-week-old C57BL/6J mice, after culture with M-CSF for 3 days and then with RANKL or RANKL plus exogenous CD200 for another 5 days. g Quantitation of the percentage of resorption in four independent experiments. h Real-time PCR of hedgehog signalling members ( Ihh , Ptch1 , and Gli1 ), osteogenic markers ( Alp , Runx2 , and Sp7 ), and osteoclastic-related markers ( CD200 , CD200R , OPG , and RANKL ) in the suture mesenchyme of C57BL/6J mice 2 weeks post treatment with or without GDC0449 (hedgehog inhibitor) from five independent samples. T tests were performed. * P < 0.05; ** P < 0.01. Scale bars, 100 µm
Na Plastic Equipment Greiner Bio One 6 Well Culture Plate, supplied by Greiner Bio, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Upregulated CD200 may contribute to decreased osteoclastogenic activity in Gli1-Cre ERT2 ;Bmpr1a fl/fl sutures. a FACS analysis of CD200 in suture mesenchymal cells collected from Bmpr1α fl/fl (control) and Gli1-Cre ERT2 ;Bmpr1α fl/fl (mutant) mice 2 weeks post induction. b Quantitation of the percentages of CD200+ cells in suture mesenchyme from three independent samples. c Immunostaining of CD200 (green, indicated by arrows) in the suture mesenchyme of Bmpr1α fl/fl (control) and Gli1-Cre ERT2 ;Bmpr1α fl/fl (mutant) mice 2 weeks post induction (2wpt). Broken lines indicate the outline of the suture. d TRAP staining of osteoclasts induced from BMMs of 4-week-old C57BL/6J mice, after culture with M-CSF for 3 days and then with RANKL or RANKL plus exogenous CD200 for another 5 days. The inset shows a magnified view of the boxed region. Blue arrows indicate mature osteoclasts. e Quantitation of multinucleated TRAP+ osteoclasts per well in four independent experiments. f Resorption activity assay (von Kossa staining) of osteoclasts induced from BMMs of 4-week-old C57BL/6J mice, after culture with M-CSF for 3 days and then with RANKL or RANKL plus exogenous CD200 for another 5 days. g Quantitation of the percentage of resorption in four independent experiments. h Real-time PCR of hedgehog signalling members ( Ihh , Ptch1 , and Gli1 ), osteogenic markers ( Alp , Runx2 , and Sp7 ), and osteoclastic-related markers ( CD200 , CD200R , OPG , and RANKL ) in the suture mesenchyme of C57BL/6J mice 2 weeks post treatment with or without GDC0449 (hedgehog inhibitor) from five independent samples. T tests were performed. * P < 0.05; ** P < 0.01. Scale bars, 100 µm

Journal: Bone Research

Article Title: BMP-IHH-mediated interplay between mesenchymal stem cells and osteoclasts supports calvarial bone homeostasis and repair

doi: 10.1038/s41413-018-0031-x

Figure Lengend Snippet: Upregulated CD200 may contribute to decreased osteoclastogenic activity in Gli1-Cre ERT2 ;Bmpr1a fl/fl sutures. a FACS analysis of CD200 in suture mesenchymal cells collected from Bmpr1α fl/fl (control) and Gli1-Cre ERT2 ;Bmpr1α fl/fl (mutant) mice 2 weeks post induction. b Quantitation of the percentages of CD200+ cells in suture mesenchyme from three independent samples. c Immunostaining of CD200 (green, indicated by arrows) in the suture mesenchyme of Bmpr1α fl/fl (control) and Gli1-Cre ERT2 ;Bmpr1α fl/fl (mutant) mice 2 weeks post induction (2wpt). Broken lines indicate the outline of the suture. d TRAP staining of osteoclasts induced from BMMs of 4-week-old C57BL/6J mice, after culture with M-CSF for 3 days and then with RANKL or RANKL plus exogenous CD200 for another 5 days. The inset shows a magnified view of the boxed region. Blue arrows indicate mature osteoclasts. e Quantitation of multinucleated TRAP+ osteoclasts per well in four independent experiments. f Resorption activity assay (von Kossa staining) of osteoclasts induced from BMMs of 4-week-old C57BL/6J mice, after culture with M-CSF for 3 days and then with RANKL or RANKL plus exogenous CD200 for another 5 days. g Quantitation of the percentage of resorption in four independent experiments. h Real-time PCR of hedgehog signalling members ( Ihh , Ptch1 , and Gli1 ), osteogenic markers ( Alp , Runx2 , and Sp7 ), and osteoclastic-related markers ( CD200 , CD200R , OPG , and RANKL ) in the suture mesenchyme of C57BL/6J mice 2 weeks post treatment with or without GDC0449 (hedgehog inhibitor) from five independent samples. T tests were performed. * P < 0.05; ** P < 0.01. Scale bars, 100 µm

Article Snippet: To assess the effect of CD200, IHH, and Hh inhibitor on osteoclastogenesis, 1 μg·mL ‒1 CD200 Fc chimaera protein (R&D system, 2724-CD-050), 500 ng·mL ‒1 IHH (R&D system, 1705-HH-025), or 500 ng/ml Hh inhibitor (Selleckchem, Vismodegib (GDC0449), S1082) was added during the final 5 days of cell culture (Supplementary Fig. ).

Techniques: Activity Assay, Control, Mutagenesis, Quantitation Assay, Immunostaining, Staining, Real-time Polymerase Chain Reaction

Primer sequences used for mRNA transcript analysis

Journal: Bone Research

Article Title: BMP-IHH-mediated interplay between mesenchymal stem cells and osteoclasts supports calvarial bone homeostasis and repair

doi: 10.1038/s41413-018-0031-x

Figure Lengend Snippet: Primer sequences used for mRNA transcript analysis

Article Snippet: To assess the effect of CD200, IHH, and Hh inhibitor on osteoclastogenesis, 1 μg·mL ‒1 CD200 Fc chimaera protein (R&D system, 2724-CD-050), 500 ng·mL ‒1 IHH (R&D system, 1705-HH-025), or 500 ng/ml Hh inhibitor (Selleckchem, Vismodegib (GDC0449), S1082) was added during the final 5 days of cell culture (Supplementary Fig. ).

Techniques: Sequencing